| Title: | Assessment of genetic diversity and volatile content of commercially grown banana (Musa spp.) cultivars |
| Author(s): | Hinge VR; Shaikh IM; Chavhan RL; Deshmukh AS; Shelake RM; Ghuge SA; Dethe AM; Suprasanna P; Kadam US; |
| Address: | "Department of Plant Biotechnology, Vilasrao Deshmukh College of Agricultural Biotechnology (Vasantrao Naik Marathwada Agricultural University, Parbhani), Latur, Maharashtra, India. Division of Applied Life Science, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, Gyeongnam, 52828, Republic of Korea. Agricultural Research Organization (ARO), The Volcani Institute, P. O. Box 15159, 7505101, Rishon LeZion, Israel. Homi Bhabha National Institute (HBNI) and Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Center, Mumbai, India. Department of Plant Biotechnology, Vilasrao Deshmukh College of Agricultural Biotechnology (Vasantrao Naik Marathwada Agricultural University, Parbhani), Latur, Maharashtra, India. ulhasskadam@gmail.com. Division of Applied Life Science, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, Gyeongnam, 52828, Republic of Korea. ulhasskadam@gmail.com" |
| DOI: | 10.1038/s41598-022-11992-1 |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Banana is an important fruit crop in the tropics and subtropics; however, limited information on biomarkers and signature volatiles is available for selecting commercial cultivars. Clonal fidelity is a major contributor to banana yield and aroma; however, there are no useful biomarkers available to validate clonal fidelity. In this study, we performed the molecular profiling of 20 banana cultivars consisting of diploid (AA or AB) and triploid (AAA or AAB or ABB) genomic groups. We screened 200 molecular markers, of which 34 markers (11 RAPD, 11 ISSR, and 12 SSR) yielded unequivocally scorable biomarker profiles. About 75, 69, and 24 allelic loci per marker were detected for RAPD, ISSR, and SSR markers, respectively. The statistical analysis of molecular variance (AMOVA) exhibited a high genetic difference of 77% with a significant FST value of 0.23 (p < 0.001). Interestingly, the UBC-858 and SSR CNMPF-13 markers were unique to Grand Nain and Ardhapuri cultivars, respectively, which could be used for clonal fidelity analysis. Furthermore, the analysis of banana fruit volatilome using headspace solid-phase microextraction-gas chromatography-tandem mass spectrometry (HS-SPME-GCMS) revealed a total of fifty-four volatile compounds in nine banana cultivars with 56% of the total volatile compounds belonging to the ester group as the significant contributor of aroma. The study assumes significance with informative biomarkers and signature volatiles which could be helpful in breeding and for the authentic identification of commercial banana cultivars" |
| Keywords: | Biomarkers Gas Chromatography-Mass Spectrometry/methods Genetic Variation *Musa/chemistry/genetics Plant Breeding Random Amplified Polymorphic DNA Technique *Volatile Organic Compounds/analysis; |
| Notes: | "MedlineHinge, Vidya R Shaikh, Irfan M Chavhan, Rahul L Deshmukh, Abhijit S Shelake, Rahul Mahadev Ghuge, Sandip A Dethe, Amol M Suprasanna, Penna Kadam, Ulhas Sopanrao eng England 2022/05/14 Sci Rep. 2022 May 13; 12(1):7979. doi: 10.1038/s41598-022-11992-1" |
