| Title: | "Identification of signature volatiles to discriminate Candida albicans, glabrata, krusei and tropicalis using gas chromatography and mass spectrometry" |
| Author(s): | Hertel M; Hartwig S; Schutte E; Gillissen B; Preissner R; Schmidt-Westhausen AM; Paris S; Kastner I; Preissner S; |
| Address: | "Department of Oral Medicine, Dental Radiology and Oral Surgery, Charite - Universitatsmedizin Berlin, Berlin, Germany. Department of Oral and Maxillofacial Surgery/Clinical Navigation, Charite Universitatsmedizin Berlin, Berlin, Germany. German Cancer Consortium (DKTK), Heidelberg, Germany. Hematology, Oncology and Tumor Immunology, Charite - Universitatsmedizin Berlin, Berlin, Germany. Structural Bioinformatics Group, Institute for Physiology, Charite - Universitatsmedizin Berlin, Berlin, Germany. Department of Operative and Preventive Dentistry, Charite - Universitatsmedizin Berlin, Berlin, Germany" |
| ISSN/ISBN: | 1439-0507 (Electronic) 0933-7407 (Linking) |
| Abstract: | "Oral candidiasis is the most frequent fungal infection of the oral cavity. Clinical diagnoses require mycological confirmation, which is time-consuming in case of culture testing. The aim of the study was to identify signature volatiles to develop a chairside breath test to diagnose oral candidiasis. Headspaces above Candida albicans, glabrata, tropicalis, krusei cultures, and growth media as control were analysed after eight and 24 h using offline gas chromatography and mass spectrometry. The identification of signature volatiles was assisted using various microbial databases. Retrieved volatile patterns enabled Candida species discrimination in vitro. For C. albicans 3-methyl-2-butanone and styrene and for C. krusei a combination of p-xylene, 2-octanone, 2-heptanone and n-butyl acetate were found to be specific. 1-hexanol was found in C. tropicalis, but is emitted by a variety of other microorganisms. C. glabrata was characterised through the absence of these volatiles. The development of a breath test is a promising approach in confirming suspicions of oral candidiasis. To confirm the retrieved results in vivo, breath tests in affected and healthy subjects have to be performed" |
| Keywords: | Acetates/analysis Adult Breath Tests Candida/chemistry/*classification/*isolation & purification Candida albicans/classification/isolation & purification Candida glabrata/classification/isolation & purification Candida tropicalis/classification/isolation; |
| Notes: | "MedlineHertel, Moritz Hartwig, Stefan Schutte, Eyke Gillissen, Bernhard Preissner, Robert Schmidt-Westhausen, Andrea Maria Paris, Sebastian Kastner, Isabell Preissner, Saskia eng Germany 2015/12/17 Mycoses. 2016 Feb; 59(2):117-26. doi: 10.1111/myc.12442. Epub 2015 Dec 14" |
