| Title: | Characterization of toluene metabolism by methanotroph and its effect on methane oxidation |
| Author(s): | He R; Su Y; Ma RC; Zhuang S; |
| Address: | "Department of Environmental Engineering, Zhejiang University, Hangzhou, 310058, China. heruo@zju.edu.cn. Department of Environmental Engineering, Zhejiang University, Hangzhou, 310058, China. Institute of Environment, Resource, Soil and Fertilizer, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China. Institute of Environmental Science, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, China" |
| Journal Title: | Environ Sci Pollut Res Int |
| DOI: | 10.1007/s11356-018-1863-z |
| ISSN/ISBN: | 1614-7499 (Electronic) 0944-1344 (Linking) |
| Abstract: | "Methanotrophs not only oxidize CH(4), but also can oxidize a relatively broad range of other substrates, including trichloroethylene, alkanes, alkenes, and aromatic compounds. In this study, Methylosinus sporium was used as a model organism to characterize toluene metabolism by methanotrophs. Reverse transcription quantitative PCR analysis showed that toluene enhanced the mmoX expression of M. sporium. When the toluene concentration was below 2000 mg m(-3), the kinetics of toluene metabolism by M. sporium conformed to the Michaelis-Menten equation (V(max) = 0.238 g g(dry weight)(-1) h(-1), K (m) = 545.2 mg m(-3)). The use of a solid-phase extraction technique followed by a gas chromatography-mass spectrometry analysis and molecular docking calculation showed that toluene was likely to primarily bind the di-iron center structural region of soluble methane monooxygenase (sMMO) hydroxylase and then be oxidized to o-cresol. Although M. sporium oxidized toluene, it did not incorporate toluene into its biomass. The coexistence of toluene and CH(4) could influence CH(4) oxidation, the growth of methanotrophs, and the distribution of CH(4)-derived carbon, which were related to the ratio of the toluene concentration to biomass. These results would be helpful to understand the metabolism of CH(4) and non-methane volatile organic compounds in the environment" |
| Keywords: | Biomass Kinetics Methane/chemistry/*metabolism Molecular Docking Simulation Oxidation-Reduction Oxygenases/chemistry/*metabolism Toluene/chemistry/*metabolism Trichloroethylene/chemistry/*metabolism Methane monooxygenase Methane-derived carbon Methanotrop; |
| Notes: | "MedlineHe, Ruo Su, Yao Ma, Ruo-Chan Zhuang, Shulin eng Germany 2018/04/05 Environ Sci Pollut Res Int. 2018 Jun; 25(17):16816-16824. doi: 10.1007/s11356-018-1863-z. Epub 2018 Apr 3" |
