| Title: | Identification of odorant-binding and chemosensory protein genes and the ligand affinity of two of the encoded proteins suggest a complex olfactory perception system in Periplaneta americana |
| Author(s): | He P; Li ZQ; Zhang YF; Chen L; Wang J; Xu L; Zhang YN; He M; |
| Address: | "State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Huaxi District, Guiyang, China. Key Laboratory of Tea Biology and Resource Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Science, Hangzhou, China. Biogas Institute of Ministry of Agriculture, Chengdu, China. The High Educational Key Laboratory of Guizhou Province for Natural Medicinal Pharmacology and Druggability, Guizhou Medical University, Guian, Guizhou, China. Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, China. College of Life Sciences, Huaibei Normal University, Huaibei, China" |
| ISSN/ISBN: | 1365-2583 (Electronic) 0962-1075 (Linking) |
| Abstract: | "The American cockroach (Periplaneta americana) is an urban pest with a precise chemosensory system that helps it achieve complex physiological behaviours, including locating food and mating. However, its chemosensory mechanisms have not been well studied. Here, we identified 71 putative odorant carrier protein genes in P. americana, including 57 new odorant-binding proteins (OBPs) and 11 chemosensory proteins (CSPs). To identify their physiological functions, we investigated their tissue expression patterns in antennae, mouthparts, legs, and the remainder of the body of both sexes, and determined that most of these genes were expressed in chemosensory organs. A phylogenetic tree showed that the putative pheromone-binding proteins of P. americana were in different clades from those of moths. Two genes, PameOBP24 and PameCSP7, were expressed equally in antennae of both sexes and highly expressed amongst the OBPs and CSPs. These genes were expressed in Escherichia coli and the resultant proteins were purified. The binding affinities of 74 common odorant compounds were tested with recombinant PameOBP24 and PameCSP7. Both proteins bound a variety of ligands. Our findings provide a foundation for future research into the chemosensory mechanisms of P. americana and help in identifying potential target genes for managing this pest" |
| Keywords: | "Animals Arthropod Antennae/metabolism Female Gene Expression Insect Proteins/*genetics/isolation & purification/metabolism Male Olfactory Perception Periplaneta/*genetics/metabolism Phylogeny Receptors, Odorant/*genetics/isolation & purification/metabolis;" |
| Notes: | "MedlineHe, P Li, Z-Q Zhang, Y-F Chen, L Wang, J Xu, L Zhang, Y-N He, M eng Research Support, Non-U.S. Gov't England 2017/07/19 Insect Mol Biol. 2017 Dec; 26(6):687-701. doi: 10.1111/imb.12328. Epub 2017 Jul 18" |
