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Photochem Photobiol Sci

Title:		"Molecular cloning, characterization, and evolution analysis of the luciferase genes from three sympatric sibling fireflies (Lampyridae: Lampyrinae, Diaphanes)"
Author(s):		He JW; Liu GC; Dong PX; Dong ZW; Zhao RP; Wang W; Li XY;
Address:		"School of Ecology and Environment, Northwestern Polytechnical University, Xi'an, 710072, Shaanxi, China. State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, Yunnan, China. Dezhou University, Dezhou, 253023, Shandong, China. School of Ecology and Environment, Northwestern Polytechnical University, Xi'an, 710072, Shaanxi, China. wwang@mail.kiz.ac.cn. State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, Yunnan, China. wwang@mail.kiz.ac.cn. State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, Yunnan, China. lixy@mail.kiz.ac.cn"
Journal Title:		Photochem Photobiol Sci
Year:		2021
Volume:		20210804
Issue:		8
Page Number:		1053 - 1067
DOI:		10.1007/s43630-021-00080-4
ISSN/ISBN:		1474-9092 (Electronic) 1474-905X (Linking)
Abstract:		"Firefly adult bioluminescence functions as signal communication between sexes. How sympatric sibling species with similar glow pattern recognize their conspecific mates remains largely unknown. To better understand the role of the luciferases of sympatric fireflies in recognizing mates, we cloned the luciferase genes of three sympatric forest dwelling fireflies (Diaphanes nubilus, Diaphanes pectinealis, and Diaphanes sp2) and evaluated their enzyme characteristics. Our data show that the amino acid (AA) sequences of all three luciferases are highly conserved, including the identities (D. nubilus vs D. pectinealis: 99%; D. nubilus vs Diaphanes sp2: 98.5%; D. pectinealis vs Diaphanes sp2: 99.4%) and the protein structures. Three recombinant luciferases produced in vitro all possess significant luminescence activity at pH 7.8, and similar maximum emission spectrum (D. nubilus: 562 nm; D. pectinealis and Diaphanes sp2: 564 nm). They show the highest activity at 10 degrees C (D. pectinealis, Diaphanes sp2) and 15 degrees C (D. nubilus), and completely inactivation at 45 degrees C. Their K(M) for D-luciferin and ATP were 2.7 muM and 92 muM (D. nubilus), 3.7 muM and 49 muM (D. pectinealis), 3.5 muM and 46 muM (Diaphanes sp2). Phylogenetic analyses support that D. nubilus is sister to D. pectinealis with Diaphanes sp2 at their base, which further cluster with Pyrocoelia. All combined data indicate that sympatric Diaphanes species have similar luciferase characteristics, suggesting that other strategies (e.g., pheromone, active time, etc.) may be adopted to recognize mates. Our data provide new insights into Diaphanes luciferases and their evolution"
Keywords:		"Animals Cloning, Molecular *Evolution, Molecular Fireflies/*genetics Luciferases/*genetics *Sympatry;"
Notes:		"MedlineHe, Jin-Wu Liu, Gui-Chun Dong, Ping-Xuan Dong, Zhi-Wei Zhao, Ruo-Ping Wang, Wen Li, Xue-Yan eng 2014FB179/Yunnan Provincial Science and Technology Department/ 202101AS070054/yunnan provincial science and technology department/ 202105AC160039/yunnan provincial science and technology department/ 31472035/National Natural Science Foundation of China/ England 2021/08/05 Photochem Photobiol Sci. 2021 Aug; 20(8):1053-1067. doi: 10.1007/s43630-021-00080-4. Epub 2021 Aug 4"