| Title: | Light dominates the diurnal emissions of herbivore-induced volatiles in wild tobacco |
| Author(s): | He J; Halitschke R; Schuman MC; Baldwin IT; |
| Address: | "National Citrus Engineering Research Center, Citrus Research Institute, Southwest University, Xiema Street, Beibei, Chongqing, 400712, People's Republic of China. hejunyer@outlook.com. Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Hans-Knoll-Strasse 8, 07745, Jena, Germany. hejunyer@outlook.com. Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Hans-Knoll-Strasse 8, 07745, Jena, Germany. Current address: Departments of Geography and Chemistry, University of Zurich, 8057, Zurich, Switzerland. Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Hans-Knoll-Strasse 8, 07745, Jena, Germany. baldwin@ice.mpg.de" |
| DOI: | 10.1186/s12870-021-03179-z |
| ISSN/ISBN: | 1471-2229 (Electronic) 1471-2229 (Linking) |
| Abstract: | "BACKGROUND: Timing is everything when it comes to the fitness outcome of a plant's ecological interactions, and accurate timing is particularly relevant for interactions with herbivores or mutualists that are based on ephemeral emissions of volatile organic compounds. Previous studies of the wild tobacco N. attenuata have found associations between the diurnal timing of volatile emissions, and daytime predation of herbivores by their natural enemies. RESULTS: Here, we investigated the role of light in regulating two biosynthetic groups of volatiles, terpenoids and green leaf volatiles (GLVs), which dominate the herbivore-induced bouquet of N. attenuata. Light deprivation strongly suppressed terpenoid emissions while enhancing GLV emissions, albeit with a time lag. Silencing the expression of photoreceptor genes did not alter terpenoid emission rhythms, but silencing expression of the phytochrome gene, NaPhyB1, disordered the emission of the GLV (Z)-3-hexenyl acetate. External abscisic acid (ABA) treatments increased stomatal resistance, but did not truncate the emission of terpenoid volatiles (recovered in the headspace). However, ABA treatment enhanced GLV emissions and leaf internal pools (recovered from tissue), and reduced internal linalool pools. In contrast to the pattern of diurnal terpenoid emissions and nocturnal GLV emissions, transcripts of herbivore-induced plant volatile (HIPV) biosynthetic genes peaked during the day. The promotor regions of these genes were populated with various cis-acting regulatory elements involved in light-, stress-, phytohormone- and circadian regulation. CONCLUSIONS: This research provides insights into the complexity of the mechanisms involved in the regulation of HIPV bouquets, a mechanistic complexity which rivals the functional complexity of HIPVs, which includes repelling herbivores, calling for body guards, and attracting pollinators" |
| Keywords: | "Abscisic Acid/pharmacology Animals *Circadian Rhythm Gene Expression Regulation, Plant/drug effects/physiology Herbivory/*physiology Larva/physiology *Light Moths/physiology Phytochrome B/genetics/metabolism Plant Proteins/genetics/metabolism Terpenes/met;" |
| Notes: | "MedlineHe, Jun Halitschke, Rayko Schuman, Meredith C Baldwin, Ian T eng England 2021/09/01 BMC Plant Biol. 2021 Aug 30; 21(1):401. doi: 10.1186/s12870-021-03179-z" |
