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Ann Bot

Title:		Morphologies and elemental compositions of calcium crystals in phyllodes and branchlets of Acacia robeorum (Leguminosae: Mimosoideae)
Author(s):		He H; Bleby TM; Veneklaas EJ; Lambers H; Kuo J;
Address:		"School of Plant Biology, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia. heh01@student.uwa.edu.au"
Journal Title:		Ann Bot
Year:		2012
Volume:		20120131
Issue:		5
Page Number:		887 - 896
DOI:		10.1093/aob/mcs004
ISSN/ISBN:		1095-8290 (Electronic) 0305-7364 (Print) 0305-7364 (Linking)
Abstract:		"BACKGROUND AND AIMS: Formation of calcium oxalate crystals is common in the plant kingdom, but biogenic formation of calcium sulfate crystals in plants is rare. We investigated the morphologies and elemental compositions of crystals found in phyllodes and branchlets of Acacia robeorum, a desert shrub of north-western Australia. METHODS: Morphologies of crystals in phyllodes and branchlets of A. robeorum were studied using scanning electron microscopy (SEM), and elemental compositions of the crystals were identified by energy-dispersive X-ray spectroscopy. Distributional patterns of the crystals were studied using optical microscopy together with SEM. KEY RESULTS: According to the elemental compositions, the crystals were classified into three groups: (1) calcium oxalate; (2) calcium sulfate, which is a possible mixture of calcium sulfate and calcium oxalate with calcium sulfate being the major component; and (3) calcium sulfate . magnesium oxalate, presumably mixtures of calcium sulfate, calcium oxalate, magnesium oxalate and silica. The crystals were of various morphologies, including prisms, raphides, styloids, druses, crystal sand, spheres and clusters. Both calcium oxalate and calcium sulfate crystals were observed in almost all tissues, including mesophyll, parenchyma, sclerenchyma (fibre cells), pith, pith ray and cortex; calcium sulfate . magnesium oxalate crystals were only found in mesophyll and parenchyma cells in phyllodes. CONCLUSIONS: The formation of most crystals was biologically induced, as confirmed by studying the crystals formed in the phyllodes from seedlings grown in a glasshouse. The crystals may have functions in removing excess calcium, magnesium and sulfur, protecting the plants against herbivory, and detoxifying aluminium and heavy metals"
Keywords:		"Acacia/*chemistry/metabolism/ultrastructure Calcium Oxalate/*chemistry Calcium Sulfate/*chemistry Crystallization Mesophyll Cells/chemistry/metabolism/ultrastructure Microscopy, Electron, Scanning Oxalic Acid/*chemistry Plant Leaves/chemistry/metabolism/u;"
Notes:		"MedlineHe, Honghua Bleby, Timothy M Veneklaas, Erik J Lambers, Hans Kuo, John eng Research Support, Non-U.S. Gov't England 2012/02/02 Ann Bot. 2012 Apr; 109(5):887-96. doi: 10.1093/aob/mcs004. Epub 2012 Jan 31"