« Previous Abstract"Essential oils of basil chemotypes: Major compounds, binary mixtures, and antioxidant activity"    Next AbstractAnalysing small insect glands with UV-LDI MS: high-resolution spatial analysis reveals the chemical composition and use of the osmeterium secretion in Themira superba (Sepsidae: Diptera) »

Toxicol Lett

Title:		Analysis of extracellular metabolome by HS-SPME/GC-MS: Optimization and application in a pilot study to evaluate galactosamine-induced hepatotoxicity
Author(s):		Araujo AM; Moreira N; Lima AR; Bastos ML; Carvalho F; Carvalho M; Guedes de Pinho P;
Address:		"UCIBIO, REQUIMTE, Laboratory of Toxicology, Faculty of Pharmacy, University of Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313, Porto, Portugal. Electronic address: mcf11011@med.up.pt. UCIBIO, REQUIMTE, Laboratory of Toxicology, Faculty of Pharmacy, University of Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313, Porto, Portugal. UCIBIO, REQUIMTE, Laboratory of Toxicology, Faculty of Pharmacy, University of Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313, Porto, Portugal; UFP Energy, Environment and Health Research Unit (FP-ENAS), University Fernando Pessoa, Praca Nove de Abril, 349, 4249-004, Porto, Portugal. UCIBIO, REQUIMTE, Laboratory of Toxicology, Faculty of Pharmacy, University of Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313, Porto, Portugal. Electronic address: pguedes@ff.up.pt"
Journal Title:		Toxicol Lett
Year:		2018
Volume:		20180528
Issue:
Page Number:		22 - 31
DOI:		10.1016/j.toxlet.2018.05.028
ISSN/ISBN:		1879-3169 (Electronic) 0378-4274 (Linking)
Abstract:		"Two methods based on headspace solid-phase microextraction (HS-SPME) coupled to gas chromatography-mass spectrometry (GC-MS) were developed to study in vitro the volatile exometabolome, which were then further tested in a pilot study to evaluate galactosamine-induced hepatotoxicity. The analysis of volatile organic compounds (VOCs) was carried out directly in the headspace of the cell culture medium, while some other volatile organic compounds such as volatile carbonyl compounds (VCCs) (aldehydes and ketones) were determined in the headspace of the cell culture medium after a previous derivatization step with O-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine hydrochloride (PFBHA). Fiber selection was performed using a univariate mode, whereas a central composite design (CCD) was used in the optimization of several other parameters that affect the extraction conditions. VOCs showed optimal extraction results using a DVB/CAR/PDMS fiber, by adding 0.43?ª+g of NaCl to a sample volume of 2?ª+mL and allowing the sample to equilibrate for 10?ª+min at 45?ª+ degrees C with a subsequent extraction for 39?ª+min at the same temperature. For VCCs, the best extraction response was achieved after in-solution (2?ª+mL) derivatization with 0.94?ª+g?ª+L(-1) of PFBHA (final concentration), followed by an incubation period of 6?ª+min and an extraction time of 37?ª+min at 53?ª+ degrees C, using a PDMS/DVB fiber. The applicability of both optimized methods was then tested, through a untargeted study, on cell culture medium samples obtained from primary mouse hepatocytes (PMH) exposed to three low concentrations (LC(01), LC(10) and LC(30)) of the well-known hepatotoxic agent galactosamine (GalN). The results obtained by both methods showed that volatile compounds from GalN exposed cells are separated from controls in a concentration-dependent manner. Several volatile compounds, namely aldehydes, ketones and alcohols, suffered significant alterations, suggesting that GalN induces marked metabolic alterations in cells even at low, non-toxic concentrations. Although preliminary, this metabolomics approach proved its potential to be used in future studies to evaluate toxicity of different xenobiotics"
Keywords:		"Animals Biomarkers/metabolism Cells, Cultured Chemical and Drug Induced Liver Injury/etiology/*metabolism Dose-Response Relationship, Drug Galactosamine/*toxicity *Gas Chromatography-Mass Spectrometry Hepatocytes/drug effects/*metabolism Liver/drug effect;"
Notes:		"MedlineAraujo, Ana Margarida Moreira, Nathalie Lima, Ana Rita Bastos, Maria de Lourdes Carvalho, Felix Carvalho, Marcia Guedes de Pinho, Paula eng Netherlands 2018/06/01 Toxicol Lett. 2018 Oct 1; 295:22-31. doi: 10.1016/j.toxlet.2018.05.028. Epub 2018 May 28"