| Title: | Lure-and-Kill Yeast Interfering RNA Larvicides Targeting Neural Genes in the Human Disease Vector Mosquito Aedes aegypti |
| Author(s): | Hapairai LK; Mysore K; Chen Y; Harper EI; Scheel MP; Lesnik AM; Sun L; Severson DW; Wei N; Duman-Scheel M; |
| Address: | "Indiana University School of Medicine, Department of Medical and Molecular Genetics, South Bend, IN, USA. The University of Notre Dame Eck Institute for Global Health, Notre Dame, IN, USA. The University of Notre Dame Department of Civil and Environmental Engineering, Notre Dame, IN, USA. The University of Notre Dame Department of Biological Sciences, Notre Dame, IN, USA. Indiana University School of Medicine, Department of Medical and Molecular Genetics, South Bend, IN, USA. mscheel@nd.edu. The University of Notre Dame Eck Institute for Global Health, Notre Dame, IN, USA. mscheel@nd.edu. The University of Notre Dame Department of Biological Sciences, Notre Dame, IN, USA. mscheel@nd.edu" |
| DOI: | 10.1038/s41598-017-13566-y |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "New mosquito control strategies are vitally needed to address established arthropod-borne infectious diseases such as dengue and yellow fever and emerging diseases such as Zika and chikungunya, all of which are transmitted by the disease vector mosquito Aedes aegypti. In this investigation, Saccharomyces cerevisiae (baker's yeast) was engineered to produce short hairpin RNAs (shRNAs) corresponding to the Aedes aegypti orthologs of fasciculation and elongation protein zeta 2 (fez2) and leukocyte receptor cluster (lrc) member, two genes identified in a recent screen for A. aegypti larval lethal genes. Feeding A. aegypti with the engineered yeasts resulted in silenced target gene expression, disrupted neural development, and highly significant larval mortality. Larvicidal activities were retained following heat inactivation and drying of the yeast into tabular formulations that induced >95% mortality and were found to attract adult females to oviposit. These ready-to-use inactivated yeast interfering RNA tablets may one day facilitate the seamless integration of this new class of lure-and-kill species-specific biorational mosquito larvicides into integrated mosquito control programs" |
| Keywords: | "Aedes/*drug effects Animals Gene Silencing Genes, Insect Genes, Lethal Genetic Engineering Insecticides/*pharmacology Larva/*drug effects Mosquito Vectors/*drug effects Neurons/*drug effects Oviposition Pest Control, Biological RNA, Fungal/genetics RNA, S;" |
| Notes: | "MedlineHapairai, Limb K Mysore, Keshava Chen, Yingying Harper, Elizabeth I Scheel, Max P Lesnik, Alexandra M Sun, Longhua Severson, David W Wei, Na Duman-Scheel, Molly eng R21 AI128116/AI/NIAID NIH HHS/ Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S. England 2017/10/19 Sci Rep. 2017 Oct 16; 7(1):13223. doi: 10.1038/s41598-017-13566-y" |
