| Title: | A method for quantification of volatile organic compounds in blood by SPME-GC-MS/MS with broader application: From non-occupational exposure population to exposure studies |
| Author(s): | Aranda-Rodriguez R; Cabecinha A; Harvie J; Jin Z; Marchand A; Tardif R; Nong A; Haddad S; |
| Address: | "Exposure and Biomonitoring Division, Environmental Health Science and Research Bureau, 50 Columbine Driveway, Tunney's Pasture, Health Canada, Ottawa, ON K1A 0K9, Canada. Electronic address: Rocio.aranda-rodriguez@hc-sc.gc.ca. Exposure and Biomonitoring Division, Environmental Health Science and Research Bureau, 50 Columbine Driveway, Tunney's Pasture, Health Canada, Ottawa, ON K1A 0K9, Canada. Departement de sante environnementale et sante au travail, Institut de Recherche en Sante Publique, Universite de Montreal, Pavillon Marguerite d'Youville, 2375 chemin de la Cote-Sainte-Catherine, Montreal, QC H3T 1A8, Canada" |
| Journal Title: | J Chromatogr B Analyt Technol Biomed Life Sci |
| DOI: | 10.1016/j.jchromb.2015.04.020 |
| ISSN/ISBN: | 1873-376X (Electronic) 1570-0232 (Linking) |
| Abstract: | "Humans are continuously exposed to volatile organic compounds (VOCs) as these chemicals are ubiquitously present in most indoor and outdoor environments. In order to assess recent exposure to VOCs for population-based studies, VOCs are measured in the blood of participants. This work describes an improved method to detect 12 VOCs by head-space solid-phase microextraction gas chromatography coupled with isotope-dilution mass spectrometry in selected reaction monitoring mode (SPME-GC-MS/MS). This method was applied to the analysis of trihalomethanes, styrene, trichloroethylene, tetrachloroethylene and BTEX (benzene, toluene, ethylbenzene, m-xylene, p-xylene, o-xylene) in a population-based biomonitoring study (Canadian Health Measures Survey). The method showed good linearity (>0.990) in the range of 0.010-10mug/L and detection limits between 0.007 and 0.027mug/L, precision better than 25% and good accuracy (+/-25%) based on proficiency testing materials. Quality Control data among runs over a 7 month period showed %RSD between 14 and 25% at low levels ( approximately 0.03mug/L) and between 9 and 23% at high levels ( approximately 0.4mug/L). The method was modified to analyze samples from a pharmacokinetic study in which 5 healthy volunteers were exposed to single, binary and quaternary mixtures of CTEX (chloroform, ethylbenzene, toluene and m-xylene), thus the expected concentration in blood was 1 order of magnitude higher than those found in the general population. The method was modified by reducing the sample size (from 3g to 0.5g) and increasing the upper limit of the concentration range to 395mug/L. Good linearity was found in the range of 0.13-395mug/L for toluene and ethylbenzene and 0.20-609mug/L for m/p-xylene. Quality control data among runs over the period of the study (n=13) were found to vary between 7 and 25%" |
| Keywords: | Environmental Exposure/*analysis Environmental Monitoring Humans Limit of Detection Linear Models Male Reproducibility of Results Solid Phase Microextraction/*methods Tandem Mass Spectrometry/*methods Volatile Organic Compounds/*blood Biomonitoring Blood; |
| Notes: | "MedlineAranda-Rodriguez, Rocio Cabecinha, Ashley Harvie, Jeromy Jin, Zhiyun Marchand, Axelle Tardif, Robert Nong, Andy Haddad, Sami eng Research Support, Non-U.S. Gov't Netherlands 2015/05/13 J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Jun 15; 992:76-85. doi: 10.1016/j.jchromb.2015.04.020. Epub 2015 Apr 20" |
