« Previous AbstractInvasive Japanese beetles facilitate aggregation and injury by a native scarab pest of ripening fruits    Next AbstractAir pollutants emissions from waste treatment and disposal facilities »

Toxins (Basel)

Title:		"Investigation of the Occurrence of Cyanotoxins in Lake Karaoun (Lebanon) by Mass Spectrometry, Bioassays and Molecular Methods"
Author(s):		Hammoud NA; Zervou SK; Kaloudis T; Christophoridis C; Paraskevopoulou A; Triantis TM; Slim K; Szpunar J; Fadel A; Lobinski R; Hiskia A;
Address:		"National Council for Scientific Research (CNRS), P.O. Box 11-8281, Riad El Solh, Beirut 1107 2260, Lebanon. Laboratory of Photo-Catalytic Processes and Environmental Chemistry, Institute of Nanoscience and Nanotechnology, National Center for Scientific Research 'Demokritos', Patr. Grigoriou E' & 27 Neapoleos Str., Agia Paraskevi, 15341 Athens, Greece. Institut des Sciences Analytiques et de Physico-Chimie pour l'Environnement et les Materiaux, Universite de Pau et des Pays de l'Adour, E2S UPPA, CNRS, IPREM UMR 5254, Helioparc, 64053 Pau, France. Department of Water Quality Control, Athens Water Supply and Sewerage Company (EYDAP SA), 156 Oropou Str., 11146 Athens, Greece. Chemical Engineering Department, National Technical University, Iroon Politechniou 9, Zografou, 15780 Athens, Greece. Chair of Analytical Chemistry, Warsaw University of Technology, Noakowskiego 3, 00-664 Warsaw, Poland"
Journal Title:		Toxins (Basel)
Year:		2021
Volume:		20211010
Issue:		10
Page Number:		-
DOI:		10.3390/toxins13100716
ISSN/ISBN:		2072-6651 (Electronic) 2072-6651 (Linking)
Abstract:		"Lake Karaoun is the largest artificial lake in Lebanon and serves multiple purposes. Recently, intensive cyanobacterial blooms have been reported in the lake, raising safety and aesthetic concerns related to the presence of cyanotoxins and cyanobacterial taste and odor (T&O) compounds, respectively. Here, we communicate for the first time results from a recent investigation by LC-MS/MS covering multiple cyanotoxins (microcystins (MCs), anatoxin-a, cylindrospermopsin, nodularin) in water and fish collected between 2019 and 2020. Eleven MCs were identified reaching concentrations of 211 and 199 mug/L for MC-LR and MC-YR, respectively. Cylindrospermopsin, anatoxin-a and nodularin were not detected. The determination of the total MCs was also carried out by ELISA and Protein Phosphatase Inhibition Assay yielding comparable results. Molecular detection of cyanobacteria (16S rRNA) and biosynthetic genes of toxins were carried out by qPCR. Untargeted screening analysis by GC-MS showed the presence of T&O compounds, such as beta-cyclocitral, beta-ionone, nonanal and dimethylsulfides that contribute to unpleasant odors in water. The determination of volatile organic compounds (VOCs) showed the presence of anthropogenic pollutants, mostly dichloromethane and toluene. The findings are important to develop future monitoring schemes in order to assess the risks from cyanobacterial blooms with regard to the lake's ecosystem and its uses"
Keywords:		"Animals Carps Cyanobacteria/chemistry/genetics Cyanobacteria Toxins/*analysis/genetics Environmental Monitoring/methods Eutrophication Fresh Water/chemistry Lakes/*chemistry/microbiology Lebanon Odorants RNA, Ribosomal, 16S Taste Volatile Organic Compound;"
Notes:		"MedlineHammoud, Noura Alice Zervou, Sevasti-Kiriaki Kaloudis, Triantafyllos Christophoridis, Christophoros Paraskevopoulou, Aikaterina Triantis, Theodoros M Slim, Kamal Szpunar, Joanna Fadel, Ali Lobinski, Ryszard Hiskia, Anastasia eng Switzerland 2021/10/23 Toxins (Basel). 2021 Oct 10; 13(10):716. doi: 10.3390/toxins13100716"