| Title: | Cell death associated release of volatile organic sulphur compounds with antioxidant properties in chemical-challenged tobacco BY-2 suspension cultured cells |
| Author(s): | Iakimova ET; Yordanova ZP; Cristescu SM; Harren FFM; Woltering EJ; |
| Address: | "Wageningen University & Research, Horticulture and Product Physiology Group, Droevendaalsesteeg 1, P.O. Box 630, 6700AP, Wageningen, the Netherlands. Radboud University, Institute for Molecules and Materials, Department of Molecular and Laser Physics, Life Science Trace Gas Facility & Trace Gas Research Group, P.O. Box, 9010, NL-6500 GL, Nijmegen, the Netherlands. Electronic address: jiordanova@biofac.uni-sofia.bg. Radboud University, Institute for Molecules and Materials, Department of Molecular and Laser Physics, Life Science Trace Gas Facility & Trace Gas Research Group, P.O. Box, 9010, NL-6500 GL, Nijmegen, the Netherlands. Electronic address: s.cristescu@science.ru.nl. Radboud University, Institute for Molecules and Materials, Department of Molecular and Laser Physics, Life Science Trace Gas Facility & Trace Gas Research Group, P.O. Box, 9010, NL-6500 GL, Nijmegen, the Netherlands. Electronic address: f.harren@science.ru.nl. Wageningen University & Research, Horticulture and Product Physiology Group, Droevendaalsesteeg 1, P.O. Box 630, 6700AP, Wageningen, the Netherlands; Wageningen Food and Biobased Research, Bornse Weilanden 9, P.O. Box 17, 6700 AA Wageningen, the Netherlands. Electronic address: ernst.woltering@wur.nl" |
| DOI: | 10.1016/j.jplph.2020.153223 |
| ISSN/ISBN: | 1618-1328 (Electronic) 0176-1617 (Linking) |
| Abstract: | "The production of volatile organic compounds (VOCs) during programmed cell death (PCD) is still insufficiently studied and their implication in the process is not well understood. The present study demonstrates that the release of VOSCs with presumed antioxidant capacity (methanethiol, dimethylsulfide and dimethyldisulfide) accompanies the cell death in chemical-stressed tobacco BY-2 suspension cultured cells. The cells were exposed to cell death inducers of biotic nature mastoparan (MP, wasp venom) and camptothecin (CPT, alkaloid), and to the abiotic stress agent CdSO(4). The VOCs emission was monitored by proton-transfer reaction mass spectrometry (PTR-MS). The three chemicals induced PCD expressing apoptotic-like phenotype. The identified VOSCs were emitted in response to MP and CPT but not in presence of Cd. The VOSCs production occurred within few hours after the administration of the elicitors, peaked up when 20-50 % of the cells were dead and further levelled off with cell death advancement. This suggests that VOSCs with antioxidant activity may contribute to alleviation of cell death-associated oxidative stress at medium severity of cell death in response to the stress factors of biotic origin. The findings provide novel information about cell death defence mechanisms in chemical-challenged BY-2 cells and show that PCD related VOSCs synthesis depends on the type of inducer" |
| Keywords: | "Antioxidants/*metabolism Cadmium Compounds/adverse effects Camptothecin/adverse effects Cell Death/*physiology Cells, Cultured Intercellular Signaling Peptides and Proteins/adverse effects Sulfates/adverse effects Sulfur Compounds/*metabolism Tobacco/cyto;" |
| Notes: | "MedlineIakimova, Elena T Yordanova, Zhenia P Cristescu, Simona M Harren, Frans F M Woltering, Ernst J eng Germany 2020/07/10 J Plant Physiol. 2020 Aug; 251:153223. doi: 10.1016/j.jplph.2020.153223. Epub 2020 Jul 2" |
