| Title: | "Functional analysis of pheromone receptor repertoire in the fall armyworm, Spodoptera frugiperda" |
| Author(s): | Guo H; Gong XL; Li GC; Mo BT; Jiang NJ; Huang LQ; Wang CZ; |
| Address: | "State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China. CAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing, China" |
| ISSN/ISBN: | 1526-4998 (Electronic) 1526-498X (Linking) |
| Abstract: | "BACKGROUND: The fall armyworm, Spodoptera frugiperda (J. E. Smith), is a polyphagous moth species that is spreading all around the globe. It uses (Z)-9-tetradecenyl acetate (Z9-14:Ac) and (Z)-7-dodecenyl acetate (Z7-12:Ac) (100:3.9) as essential sex pheromone components. However, our understanding of the molecular basis of pheromone detection of S. frugiperda is still incomplete. RESULTS: Herein, we identified six PRs, i.e. SfruOR6, 11, 13, 16, 56, and 62, by transcriptome sequencing. Subsequently, we heterologously expressed them in Drosophila OR67d neurons and determined their response spectra with a large panel of sex pheromones and analogs. Among them, SfruOR13-expressing neurons strongly respond to the major sex pheromone component Z9-14:Ac, but also comparably to (Z,E)-9,12-tetradecadienyl acetate (Z9,E12-14:Ac) and weakly to (Z)-9-dodecenyl acetate (Z9-12:Ac). Both SfruOR56 and SfruOR62 are specifically tuned to the minor sex pheromone component Z7-12:Ac with varying intensities and sensitivities. In addition, SfruOR6 is activated only by Z9,E12-14:Ac, and SfruOR16 by both (Z)-9-tetradecenol (Z9-14:OH) and (Z)-9-tetradecenal (Z9-14:Ald). However, the OR67d neurons expressing SfruOR11 remain silent to all compounds tested, a phenomenon commonly found in the OR11 clade of Noctuidae species. Next, using single sensillum recording, we characterized four sensilla types on the antennae of males, namely A, B, C and D types that are tuned to the ligands of PRs, thereby confirming that S. frugiperda uses both SfruOR56 and SfruOR62 to detect Z7-12:Ac. Finally, using wind tunnel assay, we demonstrate that both Z9,E12-14:Ac and Z9-14:OH act as antagonists to the sex pheromone. CONCLUSION: We have deorphanized five PRs and characterized four types of sensilla responsible for the detection of pheromone compounds, providing insights into the peripheral encoding of sex pheromones in S. frugiperda" |
| Keywords: | "Animals Male *Moths Pheromones Receptors, Pheromone/genetics *Sex Attractants/pharmacology Spodoptera/genetics Drosophila OR67d neurons pheromone receptors sex pheromones single sensillum recording wind tunnel;" |
| Notes: | "MedlineGuo, Hao Gong, Xin-Lin Li, Guo-Cheng Mo, Bao-Tong Jiang, Nan-Ji Huang, Ling-Qiao Wang, Chen-Zhu eng Key Research Program of the Chinese Academy of Sciences??_KJZD-SW-L07/ 31830088/National Natural Science Foundation of China/ England 2022/02/07 Pest Manag Sci. 2022 May; 78(5):2052-2064. doi: 10.1002/ps.6831. Epub 2022 Feb 24" |
