| Title: | "Apolipophorin-III-like protein expressed in the antenna of the red imported fire ant, Solenopsis invicta Buren (Hymenoptera: Formicidae)" |
| Author(s): | Guntur KV; Velasquez D; Chadwell L; Carroll C; Weintraub S; Cassill JA; Renthal R; |
| Address: | "Department of Biology, University of Texas at San Antonio, San Antonio, Texas 78249, USA" |
| Journal Title: | Arch Insect Biochem Physiol |
| ISSN/ISBN: | 0739-4462 (Print) 0739-4462 (Linking) |
| Abstract: | "Antennal proteins of the male fire ant (Solenopsis invicta) were analyzed by two-dimensional gel electrophoresis, with the objective of identifying pheromone-binding proteins, which have not previously been found in ant antennae. The major low-molecular weight protein found in the male fire ant antenna was subjected to Edman degradation to determine the N-terminal amino acid sequence. Degenerate PCR primers based on this sequence were used to obtain a cDNA sequence corresponding to the full-length protein sequence. In-gel trypsin digestion followed by MALDI-TOF mass spectrometry and HPLC-ESI/MS/MS demonstrated that the protein gel spot contained only the protein corresponding to the cDNA sequence obtained by PCR. The sequence is similar to apolipophorin-III, an exchangeable lipid-binding protein. Fire ant apolipophorin-III is expressed in the antenna as well as the head, thorax and abdomen" |
| Keywords: | "Amino Acid Sequence Animals Ants/*genetics/*metabolism Apolipoproteins/genetics/*metabolism Base Sequence DNA Primers Electrophoresis, Gel, Two-Dimensional Male Mass Spectrometry Molecular Sequence Data Organophosphorus Compounds Reverse Transcriptase Pol;" |
| Notes: | "MedlineGuntur, Kalyani V P Velasquez, Daniel Chadwell, Linda Carroll, Christopher Weintraub, Susan Cassill, J Aaron Renthal, Robert eng Comparative Study Research Support, Non-U.S. Gov't 2004/10/16 Arch Insect Biochem Physiol. 2004 Nov; 57(3):101-10. doi: 10.1002/arch.20019" |
