| Title: | Diel Variations of Extracellular Microcystin Influence the Subcellular Dynamics of RubisCO in Microcystis aeruginosa PCC 7806 |
| Author(s): | Guljamow A; Barchewitz T; Grosse R; Timm S; Hagemann M; Dittmann E; |
| Address: | "Department of Microbiology, Institute for Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Street 24/25, 14476 Potsdam-Golm, Germany. Department of Plant Physiology, Institute of Biological Sciences, University of Rostock, Albert-Einstein-Street 3, 18059 Rostock, Germany" |
| DOI: | 10.3390/microorganisms9061265 |
| ISSN/ISBN: | 2076-2607 (Print) 2076-2607 (Electronic) 2076-2607 (Linking) |
| Abstract: | "The ubiquitous freshwater cyanobacterium Microcystis is remarkably successful, showing a high tolerance against fluctuations in environmental conditions. It frequently forms dense blooms which can accumulate significant amounts of the hepatotoxin microcystin, which plays an extracellular role as an infochemical but also acts intracellularly by interacting with proteins of the carbon metabolism, notably with the CO(2) fixing enzyme RubisCO. Here we demonstrate a direct link between external microcystin and its intracellular targets. Monitoring liquid cultures of Microcystis in a diel experiment revealed fluctuations in the extracellular microcystin content that correlate with an increase in the binding of microcystin to intracellular proteins. Concomitantly, reversible relocation of RubisCO from the cytoplasm to the cell's periphery was observed. These variations in RubisCO localization were especially pronounced with cultures grown at higher cell densities. We replicated these effects by adding microcystin externally to cultures grown under continuous light. Thus, we propose that microcystin may be part of a fast response to conditions of high light and low carbon that contribute to the metabolic flexibility and the success of Microcystis in the field" |
| Keywords: | Microcystis RubisCO cyanobacterial bloom extracellular signaling microcystin; |
| Notes: | "PubMed-not-MEDLINEGuljamow, Arthur Barchewitz, Tino Grosse, Rebecca Timm, Stefan Hagemann, Martin Dittmann, Elke eng Di910/10-1/Deutsche Forschungsgemeinschaft/ Ha2002/20-1/Deutsche Forschungsgemeinschaft/ SFB 1127/Deutsche Forschungsgemeinschaft/ GZ: INST 264/125-1 FUGG/Deutsche Forschungsgemeinschaft/ Switzerland 2021/07/03 Microorganisms. 2021 Jun 10; 9(6):1265. doi: 10.3390/microorganisms9061265" |
