| Title: | Analysis of Amygdalin in Various Matrices Using Electrospray Ionization and Flowing Atmospheric-Pressure Afterglow Mass Spectrometry |
| Author(s): | Guc M; Rutecka S; Schroeder G; |
| Address: | "Faculty of Chemistry, Adam Mickiewicz University in Poznan, Uniwersytetu Poznanskiego 8, 61-614 Poznan, Poland" |
| ISSN/ISBN: | 2218-273X (Electronic) 2218-273X (Linking) |
| Abstract: | "Amygdalin is a natural cyanogenic compound that plants produce in the fight against insects and herbivores. Excessive amounts of amygdalin by animals and humans can potentially lead to fatal intoxication. However, studies confirm that amygdalin has antitumor properties, including the ability to inhibit the proliferation of cancer cells and to induce their apoptosis. The analysis of amygdalin in various matrices is an important analytical problem today. The publication presents the methodology of direct determination of amygdalin in water, sewage, and biological materials using electrospray ionization mass spectrometry (ESI-MS) and a new analytical method using flowing atmospheric-pressure afterglow mass spectrometry (FAPA-MS). The methods of analyte pre-concentration using a magnetic, molecularly imprinted polymer (mag-MIP) and the influence of interferents on the recorded spectra were discussed. Analytical parameters in ESI-MS and FAPA-MS methods were established. The linearity range was 4.5 microg L(-1)-45 mg L(-1) in positive mode ESI-MS and FAPA-MS. The limit of detection (LOD) for ESI-MS was 0.101 +/- 0.003 microg L(-1) and the limit of quantification (LOQ) was 0.303 +/- 0.009 microg L(-1). In FAPA-MS, the LOD was 0.050 +/- 0.002 microg L(-1) and the LOQ was 0.150 +/- 0.006 microg L(-1). The content of amygdalin in various matrices was determined" |
| Keywords: | "Amygdalin/chemistry/*isolation & purification Animals Antineoplastic Agents/chemistry/*isolation & purification/therapeutic use Apoptosis/drug effects Atmospheric Pressure Cell Proliferation/drug effects Chromatography, High Pressure Liquid Humans Limit o;" |
| Notes: | "MedlineGuc, Maria Rutecka, Sandra Schroeder, Grzegorz eng 2016/21/B/ST4/02082/Narodowym Centrum Nauki/International POWR.03.02.00-00-I023/17/Narodowe Centrum Badan i Rozwoju/International Research Support, Non-U.S. Gov't Switzerland 2020/10/23 Biomolecules. 2020 Oct 19; 10(10):1459. doi: 10.3390/biom10101459" |
