| Title: | Functional characterizations of chemosensory proteins of the alfalfa plant bug Adelphocoris lineolatus indicate their involvement in host recognition |
| Author(s): | Gu SH; Wang SY; Zhang XY; Ji P; Liu JT; Wang GR; Wu KM; Guo YY; Zhou JJ; Zhang YJ; |
| Address: | "State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China" |
| DOI: | 10.1371/journal.pone.0042871 |
| ISSN/ISBN: | 1932-6203 (Electronic) 1932-6203 (Linking) |
| Abstract: | "Insect chemosensory proteins (CSPs) have been proposed to capture and transport hydrophobic chemicals from air to olfactory receptors in the lymph of antennal chemosensilla. They may represent a new class of soluble carrier protein involved in insect chemoreception. However, their specific functional roles in insect chemoreception have not been fully elucidated. In this study, we report for the first time three novel CSP genes (AlinCSP1-3) of the alfalfa plant bug Adelphocoris lineolatus (Goeze) by screening the antennal cDNA library. The qRT-PCR examinations of the transcript levels revealed that all three genes (AlinCSP1-3) are mainly expressed in the antennae. Interestingly, these CSP genes AlinCSP1-3 are also highly expressed in the 5(th) instar nymphs, suggesting a proposed function of these CSP proteins (AlinCSP1-3) in the olfactory reception and in maintaining particular life activities into the adult stage. Using bacterial expression system, the three CSP proteins were expressed and purified. For the first time we characterized the types of sensilla in the antennae of the plant bug using scanning electron microscopy (SEM). Immunocytochemistry analysis indicated that the CSP proteins were expressed in the pheromone-sensitive sensilla trichodea and general odorant-sensitive sensilla basiconica, providing further evidence of their involvement in chemoreception. The antennal activity of 55 host-related semiochemicals and sex pheromone compounds in the host location and mate selection behavior of A. lineolatus was investigated using electroantennogram (EAG), and the binding affinities of these chemicals to the three CSPs (AlinCSP1-3) were measured using fluorescent binding assays. The results showed several host-related semiochemicals, (Z)-3-hexen-1-ol, (E)-2-hexen-1-al and valeraldehyde, have a high binding affinity with AlinCSP1-3 and can elicit significant high EAG responses of A. lineolatus antennae. Our studies indicate the three antennae-biased CSPs may mediate host recognition in the alfalfa plant bug A. lineolatus" |
| Keywords: | "Amino Acid Sequence Animals Arthropod Antennae/metabolism/ultrastructure Base Sequence DNA, Complementary/chemistry Female Gene Expression Profiling Heteroptera/genetics/*metabolism/ultrastructure Host-Pathogen Interactions Insect Proteins/chemistry/genet;" |
| Notes: | "MedlineGu, Shao-Hua Wang, Song-Ying Zhang, Xue-Ying Ji, Ping Liu, Jing-Tao Wang, Gui-Rong Wu, Kong-Ming Guo, Yu-Yuan Zhou, Jing-Jiang Zhang, Yong-Jun eng Research Support, Non-U.S. Gov't 2012/08/18 PLoS One. 2012; 7(8):e42871. doi: 10.1371/journal.pone.0042871. Epub 2012 Aug 10" |
