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Br J Nutr

Title:		Effects of dietary coconut oil on apolipoprotein B synthesis and VLDL secretion by calf liver slices
Author(s):		Gruffat-Mouty D; Graulet B; Durand D; Samson-Bouma ME; Bauchart D;
Address:		"Unite de Recherches sur les Herbivores, Equipe Nutriments et Metabolismes, Institut National de Recherche Agronomique, Centre de Recherches Clermont Ferrand - Theix, 63122 Saint Genes Champanelle, France. gruffat@clermont.inra.fr"
Journal Title:		Br J Nutr
Year:		2001
Volume:		86
Issue:		1
Page Number:		13 - 19
DOI:		10.1079/bjn2001361
ISSN/ISBN:		0007-1145 (Print) 0007-1145 (Linking)
Abstract:		"Incorporation of coconut oil (CO) rich in lauric acid into the milk diet induces a lipid infiltration of the liver (steatosis) in 1-month-old calves. Among possible steps involved in diet-induced liver steatosis, the ability of the calf liver to synthesize apolipoprotein (Apo) B and to secrete it as part of VLDL particles was investigated. Liver samples were taken from calves fed for 17 d on a conventional milk replacer containing CO (n 5) and beef tallow (BT, n 4) as reference. Samples were cut into slices 0.5 mm thick and subsequently incubated for 12 h in a medium containing a [(35)S]methionine-[(35)S]cysteine mix and 0.8 mm-sodium laurate or oleate, the major fatty acids of CO and BT diets respectively. Concentrations of total [(35)S]proteins, [(35)S]albumin and [(35)S]ApoB in liver cells were 2-fold lower 0.0004 and 0.03 respectively) in CO- than in BT-fed calves. Although the total amount of proteins secreted (including albumin) was similar in both groups of calves, the amount of VLDL-[(35)S]Apo secreted was 2-fold lower (P = 0.004) in CO- than in BT-fed calves. These results suggest that a CO-enriched milk diet induces in preruminant calves a lipid infiltration of the liver by decreasing ApoB synthesis, leading to a reduction in secretion of VLDL particles"
Keywords:		"Animals Apolipoproteins B/*biosynthesis Biological Availability Cattle Cattle Diseases/chemically induced/*metabolism Coconut Oil Culture Techniques Dietary Fats/administration & dosage Fatty Liver/veterinary Lipoproteins, VLDL/*metabolism Liver/*metaboli;"
Notes:		"MedlineGruffat-Mouty, D Graulet, B Durand, D Samson-Bouma, M E Bauchart, D eng Comparative Study England 2001/07/04 Br J Nutr. 2001 Jul; 86(1):13-9. doi: 10.1079/bjn2001361"