Title: | Antioxidant activities of the supercritical and conventional Satureja montana extracts |
Author(s): | Grosso C; Oliveira AC; Mainar AM; Urieta JS; Barroso JG; Palavra AM; |
Address: | "Dept. de Engenharia Quimica e Biologica, IST, Av. Rovisco Pais, 1, 1049-001 Lisboa, Portugal. clara.f.grosso@ist.utl.pt" |
DOI: | 10.1111/j.1750-3841.2009.01376.x |
ISSN/ISBN: | 1750-3841 (Electronic) 0022-1147 (Linking) |
Abstract: | "The antioxidant activities of the volatile and the nonvolatile fractions from Satureja montana obtained by supercritical fluid extraction (SFE) and by conventional techniques, hydrodistillation (HD) and soxhlet extraction (SE), were compared. A good agreement between DPPH and rancimat methods was obtained showing that the extracts were able to scavenge free radicals and to inhibit lipid oxidation. The volatile oil (obtained by SFE at 90 bar/40 degrees C) was the most effective extract, presenting the lowest EC(50) (0.06 g/L) and the highest protector factor (PF = 2.03). These results demonstrated the advantages of SFE over conventional techniques by avoiding thermodegradation and hydrolysis reactions. Furthermore, volatile oil is 15 times richer in thymoquinone than the essential oil (HD). This compound is of great importance due to its antioxidant, neuroprotective, and anti-cancer activities. The combination of carvacrol + thymol + thymoquinone in volatile oil may be responsible for the increase in the antioxidant activity when compared to HD, which demonstrates that, in this case, SFE improves value to the final product" |
Keywords: | "Antioxidants/analysis/*chemistry/isolation & purification Benzoquinones/analysis Chemical Fractionation/*methods Cymenes Fatty Acids/analysis Fatty Acids, Nonesterified/analysis Food Preservatives/chemistry/isolation & purification Monoterpenes/analysis O;" |
Notes: | "MedlineGrosso, C Oliveira, A C Mainar, A M Urieta, J S Barroso, J G Palavra, A M F eng Comparative Study Research Support, Non-U.S. Gov't Validation Study 2010/05/25 J Food Sci. 2009 Nov-Dec; 74(9):C713-7. doi: 10.1111/j.1750-3841.2009.01376.x" |