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Int J Food Microbiol

Title:		A comparative study on the potential of epiphytic yeasts isolated from tropical fruits to produce flavoring compounds
Author(s):		Grondin E; Shum Cheong Sing A; Caro Y; Raherimandimby M; Randrianierenana AL; James S; Nueno-Palop C; Francois JM; Petit T;
Address:		"Universite de La Reunion, Laboratoire de Chimie des Substances Naturelles et des Sciences des Aliments, LCSNSA, EA 2212, 15 Avenue Rene Cassin, 97490 Sainte Clotilde, La Reunion, France; Departement Genie Biologique, IUT, Universite de La Reunion, Saint-Pierre, Ile de La Reunion, France. Universite de La Reunion, Laboratoire de Chimie des Substances Naturelles et des Sciences des Aliments, LCSNSA, EA 2212, 15 Avenue Rene Cassin, 97490 Sainte Clotilde, La Reunion, France. Universite de La Reunion, Laboratoire de Chimie des Substances Naturelles et des Sciences des Aliments, LCSNSA, EA 2212, 15 Avenue Rene Cassin, 97490 Sainte Clotilde, La Reunion, France; Departement Agroalimentaire, ESIROI, Universite de La Reunion, Sainte-Clotilde, Ile de La Reunion, France. Laboratoire de Biotechnologie et de microbiologie, Faculte des sciences, Universite d'Antananarivo, Antananarivo 101, Madagascar. National Collection of Yeast Cultures, Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK. Universite de Toulouse, INSA, UPS, INP, LISBP, 135 Avenue de Rangueil, F-31077 Toulouse, France; INRA, UMR792 Ingenierie des Systemes Biologiques et des Procedes, F-31400 Toulouse, France; CNRS, UMR5504, F-31400 Toulouse, France 135 Avenue de Rangueil, F-31077 Toulouse, France. Universite de La Reunion, Laboratoire de Chimie des Substances Naturelles et des Sciences des Aliments, LCSNSA, EA 2212, 15 Avenue Rene Cassin, 97490 Sainte Clotilde, La Reunion, France; Departement Genie Biologique, IUT, Universite de La Reunion, Saint-Pierre, Ile de La Reunion, France. Electronic address: thomas.petit@univ-reunion.fr"
Journal Title:		Int J Food Microbiol
Year:		2015
Volume:		20150303
Issue:
Page Number:		101 - 108
DOI:		10.1016/j.ijfoodmicro.2015.02.032
ISSN/ISBN:		1879-3460 (Electronic) 0168-1605 (Linking)
Abstract:		"In recent years, there has been an increasing interest in identifying and characterizing the yeast flora associated with diverse types of habitat because of the many potential desirable technological properties of these microorganisms, especially in food applications. In this study, a total of 101 yeast strains were isolated from the skins of tropical fruits collected in several locations in the South West Indian Ocean. Sequence analysis of the D1/D2 domains of the large subunit (LSU) ribosomal RNA gene identified 26 different species. Among them, two species isolated from the skins of Cape gooseberry and cocoa beans appeared to represent putative new yeast species, as their LSU D1/D2 sequence was only 97.1% and 97.4% identical to that of the yeasts Rhodotorula mucilaginosa and Candida pararugosa, respectively. A total of 52 Volatile Organic Compounds (VOCs) were detected by Head Space Solid Phase Micro Extraction coupled to Gas Chromatography and Mass Spectroscopy (HS-SPME-GC/MS) from the 26 yeast species cultivated on a glucose rich medium. Among these VOCs, 6 uncommon compounds were identified, namely ethyl but-2-enoate, ethyl 2-methylbut-2-enoate (ethyl tiglate), ethyl 3-methylbut-2-enoate, 2-methylpropyl 2-methylbut-2-enoate, butyl 2-methylbut-2-enoate and 3-methylbutyl 2-methylbut-2-enoate, making them possible yeast species-specific markers. In addition, statistical methods such as Principal Component Analysis allowed to associate each yeast species with a specific flavor profile. Among them, Saprochaete suaveolens (syn: Geotrichum fragrans) turned to be the best producer of flavor compounds, with a total of 32 out of the 52 identified VOCs in its flavor profile"
Keywords:		"Cluster Analysis DNA, Ribosomal/genetics Flavoring Agents/analysis *Food Microbiology Fruit/*microbiology Madagascar Reunion Tropical Climate Volatile Organic Compounds/*analysis Yeasts/*chemistry/classification/*isolation & purification/metabolism Biodiv;"
Notes:		"MedlineGrondin, Eric Shum Cheong Sing, Alain Caro, Yanis Raherimandimby, Marson Randrianierenana, Ando Lalaniaina James, Steve Nueno-Palop, Carmen Francois, Jean Marie Petit, Thomas eng Research Support, Non-U.S. Gov't Netherlands 2015/03/25 Int J Food Microbiol. 2015 Jun 16; 203:101-8. doi: 10.1016/j.ijfoodmicro.2015.02.032. Epub 2015 Mar 3"