| Title: | Isolation of a functional vesicular intermediate that mediates ER to Golgi transport in yeast |
| Author(s): | Groesch ME; Ruohola H; Bacon R; Rossi G; Ferro-Novick S; |
| Address: | "Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510-8002" |
| ISSN/ISBN: | 0021-9525 (Print) 1540-8140 (Electronic) 0021-9525 (Linking) |
| Abstract: | "We have used an in vitro assay that reconstitutes transport from the ER to the Golgi complex in yeast to identify a functional vesicular intermediate in transit to the Golgi apparatus. Permeabilized yeast cells, which serve as the donor in this assay, release a homogeneous population of vesicles that are biochemically distinct from the donor ER fraction. The isolated vesicles, containing a post-ER/pre-Golgi form of the marker protein pro-alpha-factor, were able to bind to and fuse with exogenously added Golgi membranes. The ability to isolate fusion competent vesicles provides direct evidence that ER to Golgi membrane transport is mediated by a discrete population of vesicular carriers" |
| Keywords: | "Biological Transport Cytosol/metabolism/ultrastructure Endoplasmic Reticulum/*metabolism/ultrastructure Golgi Apparatus/*metabolism/ultrastructure Kinetics Mating Factor Models, Biological Molecular Weight Organelles/*metabolism/ultrastructure Peptides/ge;" |
| Notes: | "MedlineGroesch, M E Ruohola, H Bacon, R Rossi, G Ferro-Novick, S eng CA46128/CA/NCI NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1990/07/01 J Cell Biol. 1990 Jul; 111(1):45-53. doi: 10.1083/jcb.111.1.45" |
