| Title: | Chemometric Analysis of Urinary Volatile Organic Compounds to Monitor the Efficacy of Pitavastatin Treatments on Mammary Tumor Progression over Time |
| Author(s): | Grocki P; Woollam M; Wang L; Liu S; Kalra M; Siegel AP; Li BY; Yokota H; Agarwal M; |
| Address: | "Department of Chemistry and Chemical Biology, Indiana University-Purdue University, Indianapolis, IN 46202, USA. Integrated Nanosystems Development Institute, Indiana University-Purdue University, Indianapolis, IN 46202, USA. Department of Pharmacology, School of Pharmacy, Harbin Medical University, Harbin 150081, China. Department of Biomedical Engineering, Indiana University-Purdue University, Indianapolis, IN 46202, USA. Hematology and Oncology, Ball Memorial Hospital, Indiana University Health, Muncie, IN 47303, USA. Simon Comprehensive Cancer Center, Indiana University School of Medicine, Indianapolis, IN 46202, USA. Indiana Center for Musculoskeletal Health, Indiana University School of Medicine, Indianapolis, IN 46202, USA. Biomechanics and Biomaterials Research Center, Indiana University-Purdue University, Indianapolis, IN 46202, USA. Department of Mechanical & Energy Engineering, Indiana University-Purdue University, Indianapolis, IN 46202, USA" |
| DOI: | 10.3390/molecules27134277 |
| ISSN/ISBN: | 1420-3049 (Electronic) 1420-3049 (Linking) |
| Abstract: | "Volatile organic compounds (VOCs) in urine are potential biomarkers of breast cancer. Previously, our group has investigated breast cancer through analysis of VOCs in mouse urine and identified a panel of VOCs with the ability to monitor tumor progression. However, an unanswered question is whether VOCs can be exploited similarly to monitor the efficacy of antitumor treatments over time. Herein, subsets of tumor-bearing mice were treated with pitavastatin at high (8 mg/kg) and low (4 mg/kg) concentrations, and urine was analyzed through solid-phase microextraction (SPME) coupled with gas chromatography-mass spectrometry (GC-MS). Previous investigations using X-ray and micro-CT analysis indicated pitavastatin administered at 8 mg/kg had a protective effect against mammary tumors, whereas 4 mg/kg treatments did not inhibit tumor-induced damage. VOCs from mice treated with pitavastatin were compared to the previously analyzed healthy controls and tumor-bearing mice using chemometric analyses, which revealed that mice treated with pitavastatin at high concentrations were significantly different than tumor-bearing untreated mice in the direction of healthy controls. Mice treated with low concentrations demonstrated significant differences relative to healthy controls and were reflective of tumor-bearing untreated mice. These results show that urinary VOCs can accurately and noninvasively predict the efficacy of pitavastatin treatments over time" |
| Keywords: | "Animals Chemometrics Gas Chromatography-Mass Spectrometry/methods *Mammary Neoplasms, Animal Mice Quinolines Solid Phase Microextraction/methods *Volatile Organic Compounds/analysis breast cancer biomarkers gas chromatography (GC) linear discriminant anal;" |
| Notes: | "MedlineGrocki, Paul Woollam, Mark Wang, Luqi Liu, Shengzhi Kalra, Maitri Siegel, Amanda P Li, Bai-Yan Yokota, Hiroki Agarwal, Mangilal eng 1852105/National Science Foundation/ 1502310/National Science Foundation/ R01AR052144/National Institute of Health (NIH)/National Institute of Arthritis and Musculoskeletal and Skin Diseases/ Switzerland 2022/07/10 Molecules. 2022 Jul 3; 27(13):4277. doi: 10.3390/molecules27134277" |
