| Title: | "Identification, functional characterization, and regulation of the enzyme responsible for floral (E)-nerolidol biosynthesis in kiwifruit (Actinidia chinensis)" |
| Author(s): | Green SA; Chen X; Nieuwenhuizen NJ; Matich AJ; Wang MY; Bunn BJ; Yauk YK; Atkinson RG; |
| Address: | "The New Zealand Institute for Plant & Food Research Limited, Auckland, New Zealand. sol.green@plantandfood.co.nz" |
| ISSN/ISBN: | 1460-2431 (Electronic) 0022-0957 (Print) 0022-0957 (Linking) |
| Abstract: | "Flowers of the kiwifruit species Actinidia chinensis produce a mixture of sesquiterpenes derived from farnesyl diphosphate (FDP) and monoterpenes derived from geranyl diphosphate (GDP). The tertiary sesquiterpene alcohol (E)-nerolidol was the major emitted volatile detected by headspace analysis. Contrastingly, in solvent extracts of the flowers, unusually high amounts of (E,E)-farnesol were observed, as well as lesser amounts of (E)-nerolidol, various farnesol and farnesal isomers, and linalool. Using a genomics-based approach, a single gene (AcNES1) was identified in an A. chinensis expressed sequence tag library that had significant homology to known floral terpene synthase enzymes. In vitro characterization of recombinant AcNES1 revealed it was an enzyme that could catalyse the conversion of FDP and GDP to the respective (E)-nerolidol and linalool terpene alcohols. Enantiomeric analysis of both AcNES1 products in vitro and floral terpenes in planta showed that (S)-(E)-nerolidol was the predominant enantiomer. Real-time PCR analysis indicated peak expression of AcNES1 correlated with peak (E)-nerolidol, but not linalool accumulation in flowers. This result, together with subcellular protein localization to the cytoplasm, indicated that AcNES1 was acting as a (S)-(E)-nerolidol synthase in A. chinensis flowers. The synthesis of high (E,E)-farnesol levels appears to compete for the available pool of FDP utilized by AcNES1 for sesquiterpene biosynthesis and hence strongly influences the accumulation and emission of (E)-nerolidol in A. chinensis flowers" |
| Keywords: | Actinidia/*enzymology/genetics/metabolism Acyclic Monoterpenes Alkyl and Aryl Transferases/genetics/metabolism Arabidopsis/genetics/metabolism Base Sequence Diphosphates/metabolism Diterpenes/metabolism Farnesol/analysis/*metabolism Flowers/*enzymology/ge; |
| Notes: | "MedlineGreen, Sol A Chen, Xiuyin Nieuwenhuizen, Niels J Matich, Adam J Wang, Mindy Y Bunn, Barry J Yauk, Yar-Khing Atkinson, Ross G eng Research Support, Non-U.S. Gov't England 2011/12/14 J Exp Bot. 2012 Mar; 63(5):1951-67. doi: 10.1093/jxb/err393. Epub 2011 Dec 7" |
