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Cell

Title:		a1 protein alters the DNA binding specificity of alpha 2 repressor
Author(s):		Goutte C; Johnson AD;
Address:		"Department of Biochemistry and Biophysics, University of California, San Francisco 94143"
Journal Title:		Cell
Year:		1988
Volume:		52
Issue:		6
Page Number:		875 - 882
DOI:		10.1016/0092-8674(88)90429-1
ISSN/ISBN:		0092-8674 (Print) 0092-8674 (Linking)
Abstract:		"The alpha 2 protein of S. cerevisiae, the product of the MAT alpha 2 gene, represses a set of cell-type-specific genes (the a-specific genes) by binding to an operator sequence upstream of each gene. We demonstrate that a second yeast regulatory protein, a1, the product of the MATa1 gene, can alter the binding specificity of alpha 2 so that it no longer recognizes the a-specific gene operator, but instead acquires the ability to recognize a different operator sequence found upstream of haploid-specific genes. Thus, under the influence of a1, alpha 2 can repress haploid-specific genes. An alpha cell expresses alpha 2 but not a1, so that alpha 2 turns off only the a-specific genes. An a/alpha cell makes both a1 and alpha 2, in a ratio that ensures that alpha 2 is distributed between two distinct binding modes: the alpha 2 binding mode and the a1-alpha 2 binding mode. Thus in an a/alpha cell, alpha 2 represses two distinct classes of genes"
Keywords:		"Chromosome Deletion Genes *Genes, Fungal *Genes, Regulator Mating Factor Mutation Peptides/*physiology Pheromones/*physiology Repressor Proteins/*metabolism Saccharomyces cerevisiae/*genetics Transcription Factors/*metabolism beta-Galactosidase/genetics;"
Notes:		"MedlineGoutte, C Johnson, A D eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. 1988/03/25 Cell. 1988 Mar 25; 52(6):875-82. doi: 10.1016/0092-8674(88)90429-1"